불소 민감성 Streptococcus mutans와 불소 저항성 Streptococcus mutans의 우식원성 특성 비교
Comparison of Cariogenic Characteristics between Fluoride-sensitive and Fluoride-resistant Streptococcus mutans
옹승환, 김종수, 백동헌, 유승훈,
소속 상세정보
옹승환 ( Ong Seung-Hwan ) - Dankook University College of Dentistry Department of Pediatric Dentistry
김종수 ( Kim Jong-Soo ) - Dankook University College of Dentistry Department of Pediatric Dentistry
백동헌 ( Baek Dong-Heon ) - Dankook University College of Dentistry Department of Microbiology and Immunology
유승훈 ( Yoo Seung-Hoon ) - Dankook University College of Dentistry Department of Pediatric Dentistry
Abstract
The aim of this study is to compare cariogenic characteristics of fluoride-sensitive Streptococcus mutans [fluoride-sensitive (FS) S. mutans ] and fluoride-resistant Streptococcus mutans [fluoride-resistant (FR) S. mutans] in the presence of sucrose, and to evaluate its effect on cariogenic biofilm formation. S. mutansATCC 25175 was continuously cultured in trypticase soy broth (TSB) containing NaF (70 ppm) for 40 days to generate FR S. mutans. FS and FR S. mutanswere inoculated in TSB with or without 2% sucrose, and optical density and pH were measured every hour. An oral biofilm was formed using saliva bacteria and analyzed through confocal laser scanning microscopy and CFU count. Finally, the expression of glucosyltransferases genes of both S. mutanswas investigated through RT-PCR. FR S. mutansexhibited slower growth and lower acidogenicity in the presence of sucrose compared to FS S. mutans. Both cariogenic and single species biofilm formation was lower in the presence of FR S. mutans, along with reduced number of bacteria. FR S. mutansshowed significantly low levels of gtfB, gtfC, and gtfD expression compared to FS S. mutans. On the basis of results, FR S. mutansmay be less virulent in the induction of dental caries.
키워드
Streptococcus mutans; Fluoride; Fluoride-resistance; Biofilm; Dental caries
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